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#4147 Cleaved Notch1 (Val1744) (D3B8) Rabbit mAb

CSTコード 包装
希望納入価格 (円)
国内在庫 i
2019年12月6日15時40分 現在
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#4147S100 μL66,000
#4147T20 μL39,000
Custom Antibody Sampler Kitの構成品を選択できます。

NOTCH1 製品一覧 | Notch 抗体比較 | #4147 が入っているAntibody Sampler キット一覧

感度分子量 (kDa)抗体の由来貯法
内在性110Rabbit IgG-20℃
種交差性 (社内試験済)
交差する可能性がある種 i


ヒト、マウス、ラット -
4147 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。



ウェスタンブロッティング (1:1000)免疫沈降 (1:200)クロマチン免疫沈降 (1:200)


内在性レベルのGly1753 とVal1754 (マウスのNotch1 のGly1743/Val1744 と同等) の間で開裂したNotch1 タンパク質の細胞質ドメイン (NICD) を検出します。全長あるいは他の部位で開裂したNotch1 タンパク質は認識しません。Notch1 タンパク質のC末端における変異により、細胞株によって検出するNICD のサイズは変わります。 (文献6 参照)。
ヒトのNotch1 タンパク質のVal1754 (マウスのVal1744 と同等) 開裂部位の配列 (合成ペプチド)

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※下記の社内データは、すべて4147 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Cleaved Notch1 (Val1744) (D3B8) Rabbit mAb (upper) or Notch1 (D1E11) XP® Rabbit mAb #3608 (lower).

Chromatin IP

Chromatin IP

CUTLL1 cells were cultured in media with γ-secretase inhibitor (1μM) for 3 days and then either harvested immediately (left panel) or washed and cultured in fresh media for 3h (right panel). Chromatin immunoprecipitations were performed with cross-linked chromatin from cells and Cleaved Notch1 (Val1744) (D3B8) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human HES1 promoter primers, SimpleChIP® Human HES4 Promoter Primers #7273, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.


Notch proteins (Notch1-4) are a family of transmembrane receptors that play important roles in development and the determination of cell fate (1). Mature Notch receptors are processed and assembled as heterodimeric proteins, with each dimer comprised of a large extracellular ligand-binding domain, a single-pass transmembrane domain, and a smaller cytoplasmic subunit (Notch intracellular domain, NICD) (2). Binding of Notch receptors to ligands of the Delta-Serrate-Lag2 (DSL) family triggers heterodimer dissociation, exposing the receptors to proteolytic cleavages; these result in release of the NICD, which translocates to the nucleus and activates transcription of downstream target genes (3,4).

The NICD of murine Notch1 is released (activated) by cleavage between Gly1743 and Val1744 (corresponding to Gly1753/Val1754 in human Notch1) (3, 4). Mutations that result in constitutive activation of Notch1 are associated with many different cancers, including a majority of cases of T cell acute lymphoblastic leukemia (T-ALL). Activation may be due to mutations in Notch1 itself, or in components of the ubiquitin ligase complex that negatively regulates the Notch signaling pathway (5-6).

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6883   SignalFire™ ECL Reagent
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7273   SimpleChIP® Human HES4 Promoter Primers
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9006   ChIP-Grade Protein G Magnetic Beads
9007   ChIP-Grade Protein G Agarose Beads

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Cleaved Notch1 (Val1744) (D3B8) Rabbit mAb

Immune Cell Signaling Pathways