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#41328 Histone H1.4 (D4J5Q) Rabbit mAb

CSTコード 包装
希望納入価格 (円)
国内在庫 i
2019年11月12日15時40分 現在
ご登録代理店情報 i
#41328S100 μL56,000
感度分子量 (kDa)抗体の由来貯法
内在性30Rabbit IgG-20℃
種交差性 (社内試験済)
交差する可能性がある種 i


ヒト、サル ハムスター、ウシ、イヌ
41328 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。



ウェスタンブロッティング (1:1000)クロマチン免疫沈降 (1:50)


内在性レベルのHistone H1.4 タンパク質を検出します。Histone H1.5 (UniProt P16401) とも交差します。Histones H1.1 (UniProt Q02539)、H1.2 (UniProt P16403)、H1.3 (UniProt P16402) と交差はわずかです。
ヒトのHistone H1.4 タンパク質のAla176 周辺領域 (合成ペプチド)

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※下記の社内データは、すべて41328 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T, HCT 116, and COS-7 cells using Histone H1.4 (D4J5Q) Rabbit mAb.

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells and either Histone H1.4 (D4J5Q) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human GAPDH Exon 1 Primers #5516, SimpleChIP® Human γ-Actin Promoter Primers #5037, SimpleChIP® Human RPL30 Exon 3 Primers #7014, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.


Histones are evolutionarily conserved proteins that play a vital role in the compaction, storage, and regulation of DNA within the eukaryotic nucleus. The basic subunit of chromatin, the nucleosome core particle, is composed of DNA wound around two copies each of the core histone proteins H2A, H2B, H3, and H4 (1-3). Formation of higher order chromatin structure is facilitated through the binding of linker histone H1 to the nucleosome particle (chromatosome) (4-6). In humans and mice, there are 11 distinct histone H1 variants, which include the somatic variants (H1.1, H1.2, H1.3, H1.4, and H1.5) that are expressed ubiquitously, and cell type specific variants such as H1t found in the testis and H1.0 expressed in terminally differentiated cells (6-10). Binding of histone H1 to chromatin limits accessibility of DNA to other proteins by stabilizing nucleosome positioning, competing for binding sites, and limiting the activity of chromatin remodeling proteins such as the SWI/SNF complex (6,11-14). Histone H1 binding is highly dynamic and is thought to be regulated by post-translational modifications (6). For example, cell cycle regulated phosphorylation of histone H1 leads to chromatin condensation and decondensation depending on the site of phosphorylation and histone H1.4 Lys34 acetylation by GCN5 has been linked to increased mobility of H1.4 and transcriptional activation (6, 15-18).

12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
4486   SimpleChIP® Human α Satellite Repeat Primers
5037   SimpleChIP® Human γ-Actin Promoter Primers
5516   SimpleChIP® Human GAPDH Exon 1 Primers
6883   SignalFire™ ECL Reagent
7014   SimpleChIP® Human RPL30 Exon 3 Primers
7017   6-Tube Magnetic Separation Rack
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
9002   SimpleChIP® Enzymatic Chromatin IP Kit (Agarose Beads)
9003   SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads)
9004   SimpleChIP® Plus Enzymatic Chromatin IP Kit (Agarose Beads)
9005   SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads)
9006   ChIP-Grade Protein G Magnetic Beads
9007   ChIP-Grade Protein G Agarose Beads

Tween is a registered trademark of ICI Americas, Inc.
SimpleChIP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.


Histone H1.4 (D4J5Q) Rabbit mAb

Metabolic Reprogramming in Disease