#4108 LC3A/B Antibody
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CSTコード | 包装 | 希望納入価格 (円) |
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#4108S | 100 μL | 56,000 | ログインすると国内在庫状況がご確認いただけます。
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感度 | 分子量 (kDa) | 抗体の由来 | 貯法 | |
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内在性 | 14, 16 | Rabbit | -20℃ |
種交差性 (社内試験済) | |
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ヒト、マウス、ラット | サル、ニワトリ、アフリカツメガエル、ゼブラフィッシュ、イヌ |
ウェスタンブロッティング (1:1000)、免疫蛍光細胞染色 (IF-IC) (1:200)、フローサイトメトリー (1:50)
特異性・感度 | |
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内在性レベルのLC3A とLC3B タンパク質を検出します。LC3C タンパク質と交差反応する可能性があります。LC3A/B タンパク質のType Ⅱ 型に強い反応性を示します。 |
使用抗原 | |
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LC3B タンパク質のGly40 周辺領域 (合成ペプチド) |
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社内データ |
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※下記の社内データは、すべて4108 の推奨プロトコールで実験した結果です。
Western Blotting

Western blot analysis of extracts from various cell lines, untreated or treated with chloroquine (50 μM, overnight) using LC3A/B Antibody.
IF-IC

Confocal immunofluorescent analysis of HCT-116 cells, untreated (left) or choroquine-treated (50 uM, overnight; right) using LC3A/B Antibody (green) and β-Catenin (L54E2) Mouse mAb (Alexa Fluor® 555 Conjugate) #5612 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Flow Cytometry

Flow cytometric analysis of Hela cells using LC3A/B Antibody (blue) compared to a nonspecific negative control antibody (red).
バックグラウンド |
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Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation, but it has also been associated with a number of physiological processes including development, differentiation, neurodegenerative diseases, infection, and cancer (3). Autophagy marker Light Chain 3 (LC3) was originally identified as a subunit of microtubule-associated proteins 1A and 1B (termed MAP1LC3) (4) and subsequently found to contain similarity to the yeast protein Apg8/Aut7/Cvt5 critical for autophagy (5). Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo post-translational modifications during autophagy (6-9). Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form. During autophagy, LC3-I is converted to LC3-II through lipidation by a ubiquitin-like system involving Atg7 and Atg3 that allows for LC3 to become associated with autophagic vesicles (6-10). The presence of LC3 in autophagosomes and the conversion of LC3 to the lower migrating form, LC3-II, have been used as indicators of autophagy (11).
- Reggiori, F. and Klionsky, D.J. (2002) Eukaryot. Cell 1, 11-21.
- Codogno, P. and Meijer, A.J. (2005) Cell Death Differ. 12 Suppl 2, 1509-18.
- Levine, B. and Yuan, J. (2005) J. Clin. Invest. 115, 2679-88.
- Mann, S.S. and Hammarback, J.A. (1994) J. Biol. Chem. 269, 11492-97.
- Lang, T. et al. (1998) EMBO J. 17, 3597-607.
- Kabeya, Y. et al. (2000) EMBO J. 19, 5720-28.
- He, H. et al. (2003) J. Biol. Chem. 278, 29278-87.
- Tanida, I. et al. (2004) J. Biol. Chem. 279, 47704-10.
- Wu, J. et al. (2006) Biochem. Biophys. Res. Commun. 339, 437-42.
- Ichimura, Y. et al. (2000) Nature 408, 488-92.
- Kabeya, Y. et al. (2004) J. Cell Sci. 117, 2805-12.
使用例 |
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DRAQ5 is a registered trademark of Biostatus Limited.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
本製品は試験研究用です。