#3861 Phospho-FRS2-α (Tyr436) Antibody

Western Blotting

Western blot analysis of extracts from COS cells cotransfected with human FRS2-alpha and rat TrkA, untreated or NGF-stimulated, using Phospho-FRS2-alpha (Tyr436) Antibody. (Cell lysates provided by Dr. Susan Meakin, Robarts Research Institute, London, Ontario.)

Fibroblast growth factor receptor substrate 2 (FRS2, also called Suc-associated neurotrophic factor-induced tyrosine-phosphorylated target or SNT) participates in the transmission of extracellular signals from the fibroblast growth factor receptor (FGFR). Activation of the FGFR leads to tyrosine phosphorylation of FRS2 (1). Two FRS2 family members have been identified, FRS2-alpha (SNT1) and FRS2-beta (SNT2) (2), which are phosphorylated by these RTKs. Once they are phosphorylated, they recruit SH2 domain-containing proteins including Grb2 and SHP-2 (3,4), mediating downstream signaling. Tyr436 is required for efficient SHP-2 recruitment (5), whereas Tyr196 functions as a docking site for Grb2-Sos complexes (6).

1. Kouhara, H. et al. (1997) Cell 89, 693-702.
2. Ong, S. H. et al. (2000) Mol. Cell. Biol. 20, 979-989.
3. Kontaridis, M. I. et al. (2002) Mol. Cell. Biol. 22, 3875-3891.
4. Xu, H. and Goldfarb, M. (2001) J. Biol. Chem. 276, 13049-13056.
5. Hadari, Y. R. et al. (1998) Mol. Cell. Biol. 18, 3966-3973.
6. Kouhara, M. et al. (1997) Cell 89, 693-702.