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#3817 Phospho-VEGF Receptor 2 (Tyr1059) (D5A6) Rabbit mAb

CSTコード 包装
希望納入価格 (円)
国内在庫 i
2019年11月13日15時20分 現在
ご登録代理店情報 i
#3817S100 μL66,000

VEGFR1 製品一覧 | #3817 が入っているAntibody Sampler キット一覧

感度分子量 (kDa)抗体の由来貯法
内在性230Rabbit IgG-20℃
種交差性 (社内試験済)
交差する可能性がある種 i


ヒト、マウス ラット
3817 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。



ウェスタンブロッティング (1:1000)


内在性レベルのTyr1059 がリン酸化されたVEGFR2 タンパク質を検出します。VEGFR1、2、3 はエピトープ領域内に同じ配列を共有しているため、相同するチロシン残基がリン酸化されたVEGFR1、3 を検出する可能性があります。
ヒトのVEGFR2 タンパク質のTyr1059 周辺領域 (合成リン酸化ペプチド)

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※下記の社内データは、すべて3817 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of PAE/CKR cells, untreated or stimulated with CSF-1, using Phospho-VEGF Receptor 2 (Tyr1059) (D5A6) Rabbit mAb (upper) and VEGF Receptor 2 (55B11) Rabbit mAb #2479 (lower). PAE/CKR cells express a chimeric receptor made up of human CSF-1 receptor extracellular domain and mouse VEGF receptor 2 transmembrane and intracellular domains.


Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor for VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase catalytic domain (Tyr1054/1059) (2). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, GRB2, PI3 kinase, NCK, and the protein tyrosine phosphatases SHP-1 and SHP-2 (3). Phosphorylation at Tyr1212 provides a docking site for GRB2 binding and phospho-Tyr1175 binds the p85 subunit of PI3 kinase and PLCγ, as well as Shb (1,4,5). Signaling from VEGFR2 is necessary for the execution of VEGF-stimulated proliferation, chemotaxis and sprouting, as well as survival of cultured endothelial cells in vitro and angiogenesis in vivo (6-8).

12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
2445   VEGF-C Antibody
2463   VEGF-B Antibody
2471   Phospho-VEGF Receptor 2 (Tyr951) Antibody
2472   VEGF Receptor 2 Antibody
2474   Phospho-VEGF Receptor 2 (Tyr996) Antibody
2476   Phospho-VEGF Receptor 2 (Tyr951) (7H11) Mouse mAb
2477   Phospho-VEGF Receptor 2 (Tyr1212) (11A3) Rabbit mAb
2478   Phospho-VEGF Receptor 2 (Tyr1175) (19A10) Rabbit mAb
2479   VEGF Receptor 2 (55B11) Rabbit mAb
2485   VEGF Receptor 3 Antibody
2638   VEGF Receptor 3 (C28G5) Rabbit mAb
2893   VEGF Receptor 1 Antibody
2904   VEGF Receptor 2 Control Proteins
3770   Phospho-VEGF Receptor 2 (Tyr1175) (D5B11) Rabbit mAb
4991   Phospho-VEGF Receptor 2 (Tyr951) (15D2) Rabbit mAb
6883   SignalFire™ ECL Reagent
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
8065   Human Vascular Endothelial Growth Factor-165 (hVEGF165 )

Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.


Phospho-VEGF Receptor 2 (Tyr1059) (D5A6) Rabbit mAb

Immune Cell Signaling Pathways