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#37988 Mouse (E1D5H) mAb IgG3 Isotype Control

CSTコード 包装
希望納入価格 (円)
国内在庫 i
2019年11月13日15時20分 現在
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#37988S100 μL25,000
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抗体の由来貯法
Mouse IgG3-20℃
37988 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。

注:各製品に最適化されたプロトコールをリンクしています。

 

免疫沈降 (-)クロマチン免疫沈降 (-)

CST推奨プロトコールに欠かせない関連製品

濃度
2.5 mg/mL
社内データ

※下記の社内データは、すべて37988 の推奨プロトコールで実験した結果です。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using Histone H3 (1B1B2) Mouse mAb #14269 (left) compared to concentration-matched Mouse (E1D5H) mAb IgG3 Isotype Control (right).

IF-IC

IF-IC

Confocal immunofluorescent analysis of SNB-19 cells using Histone H3 (1B1B2) Mouse mAb #14269 (left, green) compared to concentration-matched Mouse (E1D5H) mAb IgG3 Isotype Control (right, green). Cells were counterstained with β-Actin (13E5) Rabbit mAb #4970 (red).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of K-562 cells using Histone H3 (1B1B2) Mouse mAb #14269 (solid line) compared to concentration-matched Mouse (E1D5H) mAb IgG3 Isotype Control (dashed line). Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4408 was used as a secondary antibody.


Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 MEF cells treated with tunicamycin (2 μg/ml, 10 hr) and either Mouse (E1D5H) mAb IgG3 Isotype Control, Normal Rabbit IgG #2729, or Histone H3 (1B1B2) Mouse mAb #14269 using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR using SimpleChIP® Mouse ATF-3 Intron 1 Primers #13059, mouse CHOP promoter primers, and SimpleChIP® Mouse RPL30 Intron 2 Primers #7015. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

バックグラウンド

Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.

使用例

Alexa Fluor is a registered trademark of Life Technologies Corporation.
SimpleChIP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

Mouse (E1D5H) mAb IgG3 Isotype Control

Immune Cell Signaling Pathways

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