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#3500 SignalSilence® cdc2 siRNA I

  • SignalSilence® cdc2 siRNA I Data Sheet PDF
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希望納入価格 (円)
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2020年2月26日16時0分 現在
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#3500S300 μL57,000
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CDC2 製品一覧

種交差性機能すると推定される種
ヒトマウス
特異性
cdc2 タンパク質の発現を特異的に抑制します。
貯法
-20℃
社内データ

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® cdc2 siRNA I (+) or SignalSilence® cdc2 siRNA II #3600 (+), using cdc2 (POH1) Mouse mAb #9116 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The cdc2 (POH1) Mouse mAb confirms silencing of cdc2 expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.

バックグラウンド

The entry of eukaryotic cells into mitosis is regulated by cdc2 kinase activation, a process controlled at several steps including cyclin binding and phosphorylation of cdc2 at Thr161 (1). However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of cdc2 at Thr14 and Tyr15 (2). Phosphorylation at Thr14 and Tyr15, resulting in inhibition of cdc2, can be carried out by Wee1 and Myt1 protein kinases (3,4). The cdc25 phosphatase may be responsible for removal of phosphates at Thr14 and Tyr15 and subsequent activation of cdc2 (1,5).

使用例
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関連製品
2325   Phospho-MAPK/CDK Substrates (PXS*P or S*PXR/K) (34B2) Rabbit mAb
6201   SignalSilence® Control siRNA (Fluorescein Conjugate)
6568   SignalSilence® Control siRNA (Unconjugated)
9116   cdc2 (POH1) Mouse mAb

SignalSilence is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

SignalSilence® cdc2 siRNA I

Immune Cell Signaling Pathways

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