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#3471 Phospho-FGF Receptor (Tyr653/654) Antibody

CSTコード 包装
希望納入価格 (円)
国内在庫 i
2019年12月6日15時40分 現在
ご登録代理店情報 i
#3471S100 μL66,000

FGFR1 製品一覧

感度分子量 (kDa)抗体の由来貯法
内在性120, 145 Rabbit-20℃
種交差性 (社内試験済)
交差する可能性がある種 i


ヒト マウス、ラット
3471 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。



ウェスタンブロッティング (1:1000)


Phospho-FGF Receptor (Tyr653/654) Antibody detects endogenous levels of FGF receptors only when phosphorylated at tyrosines 653/654. This antibody detects phosphorylated FGF Receptors 2 and 4 when expressed exogenously. Based on sequence comparisons, reactivity with FGF Receptor 3 is possible but has not been experimentally confirmed. This antibody also cross-reacts with activated PDGF receptor and insulin/IGF-I receptors.
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr653/654 of human FGFR-1 (the corresponding sequence is the same in FGFR-2, -3 and -4). Antibodies are purified by protein A and peptide affinity chromatography.

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※下記の社内データは、すべて3471 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 cells overexpressing human FGFR-1, untreated or calf intestinal phosphatase (CIP)-treated, using Phospho-FGF Receptor (Tyr653/654) Antibody (upper) or FGF Receptor 1 Antibody #3472 (lower). Overexpression of human FGFR-1 protein in 293 cells results in constitutive activation of the receptors (courtesy of Dr. Pamela Maher, personal communication). CIP treatment abolishes the reactivity of this antibody to FGFR-1.

Western Blotting

Western Blotting

Western blot analysis of extracts from A-204 cells treated with or without bFGF, using Phospho-FGF Receptor (Tyr653/654) Antibody (upper) and FGF Receptor 1 (D8E4) XP® Rabbit mAb (lower).


Fibroblast growth factors (FGFs) produce mitogenic and angiogenic effects in target cells by signaling through cell surface receptor tyrosine kinases. There are four members of the FGF receptor family: FGFR1 (flg), FGFR2 (bek, KGFR), FGFR3, and FGFR4. Each receptor contains an extracellular ligand binding domain, a transmembrane domain, and a cytoplasmic kinase domain (1). Following ligand binding and dimerization, the receptors are phosphorylated at specific tyrosine residues (2). Seven tyrosine residues in the cytoplasmic tail of FGFR1 can be phosphorylated: Tyr463, 583, 585, 653, 654, 730, and 766. Tyr653 and Tyr654 are important for catalytic activity of activated FGFR and are essential for signaling (3). The other phosphorylated tyrosine residues may provide docking sites for downstream signaling components such as Crk and PLCγ (4,5).

12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
2544   Phospho-FGF Receptor 1 (Tyr766) (1E5) Rabbit mAb
3472   FGF Receptor 1 Antibody
3476   Phospho-FGF Receptor (Tyr653/654) (55H2) Mouse mAb
6883   SignalFire™ ECL Reagent
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
9740   FGF Receptor 1 (D8E4) XP® Rabbit mAb

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.


Phospho-FGF Receptor (Tyr653/654) Antibody

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