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#3393 JMJD2A (C70G6) Rabbit mAb

CSTコード 包装
希望納入価格 (円)
国内在庫 i
2020年2月28日15時15分 現在
ご登録代理店情報 i
#3393S100 μL58,000

JMJD2A 製品一覧

感度分子量 (kDa)抗体の由来貯法
内在性150Rabbit IgG-20℃
種交差性 (社内試験済)
交差する可能性がある種 i


ヒト、マウス、ラット サル
3393 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。



ウェスタンブロッティング (1:1000)免疫沈降 (1:50)免疫蛍光細胞染色 (IF-IC) (1:100)


内在性レベルのJMJD2A タンパク質を検出します。JMJD2B、JMJD2C、JMJD2Dなどの他のJumonji C タンパク質とは交差しません。
ヒトのJMJD2A タンパク質由来の配列 (合成ペプチド)

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※下記の社内データは、すべて3393 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from NCCIT, NIH/3T3 and H-4-II-E cells using JMJD2A (C70G6) Rabbit mAb.



Confocal immunofluorescent analysis of NCCIT cells using JMJD2A (C70G6) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).


The methylation state of lysine residues in histone proteins is a major determinant of the formation of active and inactive regions of the genome and is crucial for proper programming of the genome during development (1,2). Jumonji C (JmjC) domain-containing proteins represent the largest class of potential histone demethylase proteins (3). The JmjC domain can catalyze the demethylation of mono-, di-, and tri-methyl lysine residues via an oxidative reaction that requires iron and α-ketoglutarate (3). Based on homology, both humans and mice contain at least 30 such proteins, which can be divided into 7 separate families (3). The jumonji domain-containing protein 2 (JMJD2) family, also known as the JmjC domain-containing histone demethylation protein 3 (JHDM3) family, contains four members: JMJD2A/JHDM3A, JMJD2B/JHDM3B, JMJD2C/JHDM3C, and JMJD2D/JHDM3D. In addition to the JmjC domain, these proteins also contain JmjN, PHD, and tudor domains, the latter of which has been shown to bind to methylated histone H3 at Lys4 and Lys9, and methylated histone H4 at Lys20 (4,5). JMJD2 proteins have been shown to demethylate di- and tri-methyl histone H3 at Lys9 and Lys36 and function as both activators and repressors of transcription (6-11). JMJD2A, JMJD2C, and JMJD2D function as coactivators of the androgen receptor in prostate tumor cells (7). In contrast, JMJD2A also associates with Rb and NCoR corepressor complexes and is necessary for transcriptional repression of target genes (8,9). JMJD2B antagonizes histone H3 Lys9 tri-methylation at pericentric heterochromatin (10). JMJD2C, also known as GASC1, is amplified in squamous cell carcinomas and metastatic lung carcinoma and inhibition of JMJD2C expression decreases cell proliferation (11,12). JMJD2C has also been identified as a downstream target of Oct-4 and is critical for the regulation of self-renewal in embryonic stem cells (13).

12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
2184   LSD1 (C69G12) Rabbit mAb
2621   JMJD1B/JHDM2B Antibody
2901   Di-Methyl-Histone H3 (Lys36) (C75H12) Rabbit mAb
6883   SignalFire™ ECL Reagent
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
9753   Di-Methyl-Histone H3 (Lys9) Antibody
9763   Tri-Methyl-Histone H3 (Lys36) Antibody

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.


JMJD2A (C70G6) Rabbit mAb

Metabolic Reprogramming in Disease