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#2895 CHOP (L63F7) Mouse mAb

CSTコード 包装
希望納入価格 (円)
国内在庫 i
2019年11月15日15時30分 現在
ご登録代理店情報 i
#2895S100 μL56,000
#2895T20 μL39,000
Custom Antibody Sampler Kitの構成品を選択できます。

CHOP 製品一覧 | #2895 が入っているAntibody Sampler キット一覧

感度分子量 (kDa)抗体の由来貯法
内在性27Mouse IgG2a-20℃
種交差性 (社内試験済)
交差する可能性がある種 i


ヒト、マウス、ラット -
2895 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。



ウェスタンブロッティング (1:1000)免疫沈降 (1:50)免疫蛍光細胞染色 (IF-IC) (1:3200)フローサイトメトリー (1:500)クロマチン免疫沈降 (1:200)


内在性レベルのCHOP タンパク質を検出します。
ヒトのCHOP タンパク質由来の配列 (合成ペプチド)

ホモロジー (相同性) 検索をご希望の場合 >>>



※下記の社内データは、すべて2895 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from C6 and A-204 cells, untreated or treated with thapsigargin (300 nM, 2 hours) or tunicamycin (24 μg/ml, 2 hours), using CHOP (L63F7) Mouse mAb.



Confocal immunofluorescent analysis of A-204 cells, untreated (left) or tunicamycin-treated (right), using CHOP (L63F7) Mouse mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells, treated with tunicamycin (2 µg/mL, 8 hr; green) or untreated (blue) using CHOP (L63F7) Mouse mAb (solid lines) or concentration-matched Mouse (E5Y6Q) mAb IgG2a Isotype Control #61656 (dashed lines). Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4408 was used as a secondary antibody.

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from MEF wild-type cells treated with tunicamycin (2ug/ml) overnight, and CHOP (L63F7) Mouse mAb #2895 or Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Mouse ATF-3 Intron 1 Primers #13059, mouse CHOP promoter primers, and SimpleChIP® Mouse RPL30 Intron 2 Primers #7015. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.


CHOP was identified as a C/EBP-homologous protein that inhibits C/EBP and LAP in a dominant-negative manner (1). CHOP expression is induced by certain cellular stresses including starvation and the induced CHOP suppresses cell cycle progression from G1 to S phase (2). Later it was shown that, during ER stress, the level of CHOP expression is elevated and CHOP functions to mediate programmed cell death (3). Studies also found that CHOP mediates the activation of GADD34 and Ero1-Lα expression during ER stress. GADD34 in turn dephosphorylates phospho-Ser51 of eIF2α thereby stimulating protein synthesis. Ero1-Lα promotes oxidative stress inside the endoplasmic reticulum (ER) (4). The role of CHOP in the programmed cell death of ER-stressed cells is correlated with its role promoting protein synthesis and oxidative stress inside the ER (4).

12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
13059   SimpleChIP® Mouse ATF-3 Intron 1 Primers
2157   MBTPS2 Antibody
2433   Calnexin Antibody
2446   PDI Antibody
2679   Calnexin (C5C9) Rabbit mAb
2798   ERp72 Antibody
2881   ERp57 (G117) Antibody
2886   ERp44 Antibody
2887   ERp57 (A484) Antibody
2891   Calreticulin Antibody
3082   C/EBPbeta Antibody
3087   C/EBPβ (LAP) Antibody
3177   BiP (C50B12) Rabbit mAb
3183   BiP Antibody
3264   Ero1-Lα Antibody
3294   IRE1α (14C10) Rabbit mAb
3597   Phospho-eIF2α (Ser51) (119A11) Rabbit mAb
6883   SignalFire™ ECL Reagent
7015   SimpleChIP® Mouse RPL30 Intron 2 Primers
7017   6-Tube Magnetic Separation Rack
7076   Anti-mouse IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
9002   SimpleChIP® Enzymatic Chromatin IP Kit (Agarose Beads)
9003   SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads)
9004   SimpleChIP® Plus Enzymatic Chromatin IP Kit (Agarose Beads)
9005   SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads)
9006   ChIP-Grade Protein G Magnetic Beads
9007   ChIP-Grade Protein G Agarose Beads
9721   Phospho-eIF2α (Ser51) Antibody
9956   ER Stress Antibody Sampler Kit
9997   Tris Buffered Saline with Tween® 20 (TBST-10X)
9999   Nonfat Dry Milk

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CHOP (L63F7) Mouse mAb

Immune Cell Signaling Pathways