#2653 Stat4 (C46B10) Rabbit mAb
|Stat4 タンパク質のLys151 周辺領域 (合成ペプチド)|
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Western blot analysis of extracts from various cell lines and from rat thymus using Stat4 (C46B10) Rabbit mAb.
Western blot analysis of extracts from ACHN, SR and Caki cell lines and from rat thymus using Stat4 (C46B10) Rabbit mAb.
Immunoprecipitation of Stat4 protein from NK-92 cell extracts. Lane 1 is 10% input, lane 2 is beads only, lane 3 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 4 is Stat4 (C46B10) Rabbit mAb. Western blot analysis was performed using Stat4 (C46B10) Rabbit mAb.
Chromatin immunoprecipitations were performed with cross-linked chromatin from NK-92 cells starved of IL-2 overnight then treated with IL-12 (10 ng/ml) for 4 hr and Stat4 (C46B10) Rabbit mAb, using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Libraries were prepared using SimpleChIP® ChIP-seq DNA Library Prep Kit for Illumina® #56795. The figure shows binding across IRF1, a known target gene of Stat4 (see additional figure containing ChIP-qPCR data). For additional ChIP-seq tracks, please download the product data sheet.
Chromatin immunoprecipitations were performed with cross-linked chromatin from NK-92 cells starved of IL-2 overnight then treated with IL-12 (10 ng/ml) for 4 hr and either Stat4 (C46B10) Rabbit mAb #2653 or Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human IRF-1 promoter primers, human PRF1 promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
The Jak-Stat signaling pathway is utilized by a large number of cytokines, growth factors, and hormones (1). Receptor-mediated tyrosine phosphorylation of Jak family members triggers phosphorylation of Stat proteins, resulting in their nuclear translocation, binding to specific DNA elements, and subsequent activation of transcription. The remarkable range and specificity of responses regulated by the Stats is determined, in part, by the tissue-specific expression of different cytokine receptors, Jaks, and Stats, as well as by the combinatorial coupling of various Stat members to different receptors (2). Stat4 is predominantly expressed in the spleen, thymus, and testis and has been most extensively investigated as the mediator of IL-12 responses (3-8). Activation of Stat4 is associated with phosphorylation at Tyr693 (9).
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U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.