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#2641 Phospho-EGF Receptor (Tyr998) (C24A5) Rabbit mAb

CSTコード 包装
希望納入価格 (円)
国内在庫 i
2020年1月23日15時25分 現在
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#2641S100 μL67,000

EGFR 製品一覧 | EGFR 抗体比較

感度分子量 (kDa)抗体の由来貯法
内在性175Rabbit IgG-20℃
種交差性 (社内試験済)
交差する可能性がある種 i


ヒト -
2641 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。



ウェスタンブロッティング (1:1000)免疫沈降 (1:50)


内在性レベルのTyr998 がリン酸化されたEGF Receptor タンパク質を検出します。他のチロシンがリン酸化されたタンパク質とわずかに交差する可能性があります。
ヒトのEGF Receptor タンパク質のTyr998 周辺領域 (合成リン酸化ペプチド)

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※下記の社内データは、すべて2641 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from A431 and BxPC-3 cells, untreated or treated with EGF, using Phospho-EGF Receptor (Tyr998) (C24A5) Rabbit mAb (upper) and EGF Receptor Antibody #2232 (lower).


The epidermal growth factor (EGF) receptor is a transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Ligand binding results in receptor dimerization, autophosphorylation, activation of downstream signaling, internalization, and lysosomal degradation (1,2). Phosphorylation of EGF receptor (EGFR) at Tyr845 in the kinase domain is implicated in stabilizing the activation loop, maintaining the active state enzyme, and providing a binding surface for substrate proteins (3,4). c-Src is involved in phosphorylation of EGFR at Tyr845 (5). The SH2 domain of PLCγ binds at phospho-Tyr992, resulting in activation of PLCγ-mediated downstream signaling (6). Phosphorylation of EGFR at Tyr1045 creates a major docking site for the adaptor protein c-Cbl, leading to receptor ubiquitination and degradation following EGFR activation (7,8). The GRB2 adaptor protein binds activated EGFR at phospho-Tyr1068 (9). A pair of phosphorylated EGFR residues (Tyr1148 and Tyr1173) provide a docking site for the Shc scaffold protein, with both sites involved in MAP kinase signaling activation (2). Phosphorylation of EGFR at specific serine and threonine residues attenuates EGFR kinase activity. EGFR carboxy-terminal residues Ser1046 and Ser1047 are phosphorylated by CaM kinase II; mutation of either of these serines results in upregulated EGFR tyrosine autophosphorylation (10).

Phosphorylation of EGF receptor on Tyr998 was identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's LC-MS/MS platform for phosphorylation site discovery as well as another publication using MS technology (11). Phosphorylation of EGF receptor at Tyr998 was observed in select carcinoma cell lines and in tumors.

12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
2220   Phospho-EGF Receptor (Tyr1086) Antibody
2231   Phospho-EGF Receptor (Tyr845) Antibody
2232   EGF Receptor Antibody
2234   Phospho-EGF Receptor (Tyr1068) Antibody
2235   Phospho-EGF Receptor (Tyr992) Antibody
2236   Phospho-EGF Receptor (Tyr1068) (1H12) Mouse mAb
2237   Phospho-EGF Receptor (Tyr1045) Antibody
2238   Phospho-EGF Receptor (Ser1046/1047) Antibody
2239   EGF Receptor (1F4) Mouse mAb
2256   EGF Receptor (EGFR1) Mouse mAb (IP Specific)
2646   EGF Receptor (C74B9) Rabbit mAb
2963   EGF Receptor (T43) Antibody
3056   Phospho-EGF Receptor (Thr669) Antibody
3265   EGF Receptor (V279) Antibody
3777   Phospho-EGF Receptor (Tyr1068) (D7A5) XP® Rabbit mAb
4267   EGF Receptor (D38B1) XP® Rabbit mAb
4404   Phospho-EGF Receptor (Tyr1148) Antibody
4405   EGF Receptor (15F8) Rabbit mAb
4407   Phospho-EGF Receptor (Tyr1173) (53A5) Rabbit mAb
54359   EGF Receptor (D1D4J) XP® Rabbit mAb (Neutralizing)
6883   SignalFire™ ECL Reagent
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
9922   Phospho-EGF Receptor Antibody Sampler Kit

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.


Phospho-EGF Receptor (Tyr998) (C24A5) Rabbit mAb

Immune Cell Signaling Pathways