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#2616 HP1α Antibody

CSTコード 包装
希望納入価格 (円)
国内在庫 i
2019年12月13日15時35分 現在
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#2616S100 μL56,000
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HP1ALPHA 製品一覧

感度分子量 (kDa)抗体の由来貯法
内在性25 Rabbit-20℃
種交差性 (社内試験済)
交差する可能性がある種 i

社内試験はしていませんが、配列が100%相同であるため反応すると推定される種

ヒト、マウス、ラット、サル ウシ
2616 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。

注:各製品に最適化されたプロトコールをリンクしています。

 

ウェスタンブロッティング (1:1000)免疫沈降 (1:25)免疫組織染色 (パラフィン) (1:200)免疫蛍光細胞染色 (IF-IC) (1:200)フローサイトメトリー (1:100)クロマチン免疫沈降 (1:25)

下記ステップについては、データシートの右側もあわせてご参照ください。

IHC-P: 抗体希釈液 / 抗原賦活化

CST推奨プロトコールに欠かせない関連製品

特異性・感度
内在性レベルのHP1αタンパク質を検出します。HP1βおよび HP1γタンパク質とは交差しません。
使用抗原
ヒトのHP1αタンパク質のC末端領域 (合成ペプチド)

ホモロジー (相同性) 検索をご希望の場合 >>>

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社内データ

※下記の社内データは、すべて2616 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of lysates from HeLa, NIH/3T3, C6 and COS cells, using HP1alpha antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using HP1alpha Antibody in the presence of control peptide (left) or HP1 alpha blocking peptide #1004 (right).

IF-IC

IF-IC

Confocal immunofluorescent image of HeLa cells labeled with HP1α antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).


Flow Cytometry

Flow Cytometry

Flow cytometric analysis of untreated HeLa cells, using HP1alpha antibody (blue) compared to a nonspecific negative control antibody (red).

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from mES cells and either HP1α Antibody #2616 or Normal Rabbit IgG #2729, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Mouse MEST Intron 1 Primers #12870, SimpleChIP® Mouse MEST Promoter Primers #12928, mouse Intracisternal A-Particle (IAP) LTR promoter primers, and mouse primers specific to a non-genic, non-repetitive region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

バックグラウンド

Heterochromatin protein 1 (HP1) is a family of heterochromatic adaptor molecules involved in both gene silencing and higher order chromatin structure (1). All three HP1 family members (α, β, and γ) are primarily associated with centromeric heterochromatin; however, HP1β and γ also localize to euchromatic sites in the genome (2,3). HP1 proteins are approximately 25 kDa in size and contain a conserved amino-terminal chromodomain, followed by a variable hinge region and a conserved carboxy-terminal chromoshadow domain. The chromodomain facilitates binding to histone H3 tri-methylated at Lys9, a histone "mark" closely associated with centromeric heterochromatin (4,5). The variable hinge region binds both RNA and DNA in a sequence-independent manner (6). The chromoshadow domain mediates the dimerization of HP1 proteins, in addition to binding multiple proteins implicated in gene silencing and heterochromatin formation, including the SUV39H histone methyltransferase, the DNMT1 and DNMT3a DNA methyltransferases, and the p150 subunit of chromatin-assembly factor-1 (CAF1) (7-9). In addition to contributing to heterochromatin formation and propagation, HP1 and SUV39H are also found complexed with retinoblastoma (Rb) and E2F6 proteins, both of which function to repress euchromatic gene transcription in quiescent cells (10,11). HP1 proteins are subject to multiple types of post-translational modifications, including phosphorylation, acetylation, methylation, ubiquitination, and sumoylation, suggesting multiple means of regulation (12-14).

使用例
 
関連製品
1004   HP1α Blocking Peptide
12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
12870   SimpleChIP® Mouse MEST Intron 1 Primers
12928   SimpleChIP® Mouse MEST Promoter Primers
13079   Immunohistochemistry Application Solutions Kit (Rabbit)
14166   Hematoxylin
14177   SignalStain® Mounting Medium
14746   SignalStain® Citrate Unmasking Solution (10X)
15019   Animal-Free Blocking Solution (5X)
2062   Histone Deacetylase 1 (HDAC1) Antibody
2613   HP1β Antibody
2619   HP1γ Antibody
6883   SignalFire™ ECL Reagent
7017   6-Tube Magnetic Separation Rack
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
8059   SignalStain® DAB Substrate Kit
9002   SimpleChIP® Enzymatic Chromatin IP Kit (Agarose Beads)
9003   SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads)
9004   SimpleChIP® Plus Enzymatic Chromatin IP Kit (Agarose Beads)
9005   SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads)
9006   ChIP-Grade Protein G Magnetic Beads
9007   ChIP-Grade Protein G Agarose Beads
9309   Rb (4H1) Mouse mAb
9701   Phospho-Histone H3 (Ser10) Antibody
9706   Phospho-Histone H3 (Ser10) (6G3) Mouse mAb
9711   Acetyl- and Phospho-Histone H3 (Lys9/Ser10) Antibody
9715   Histone H3 Antibody
9997   Tris Buffered Saline with Tween® 20 (TBST-10X)

Alexa Fluor is a registered trademark of Life Technologies Corporation.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

HP1α Antibody

Immune Cell Signaling Pathways

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