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#2600 Phospho-HP1γ (Ser83) Antibody

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希望納入価格 (円)
国内在庫 i
2020年1月17日15時20分 現在
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#2600S100 μL67,000


感度分子量 (kDa)抗体の由来貯法
種交差性 (社内試験済)
交差する可能性がある種 i


ヒト、マウス、ラット、サル キイロショウジョウバエ、ウシ、ウマ
2600 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。



ウェスタンブロッティング (1:1000)免疫沈降 (1:25)免疫蛍光細胞染色 (IF-IC) (1:200)


内在性レベルのSer83 (プロセシングされていないHP1γタンパク質ではSer93 に相当) がリン酸化されたHP1γタンパク質を検出します。HP1αおよび HP1βタンパク質とは交差しません。
ヒトのHP1γタンパク質のSer83 周辺領域のアミノ酸 (合成リン酸化ペプチド)

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※下記の社内データは、すべて2600 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of whole cell extracts from HeLa cells, untreated (lanes 1 and 4), treated for 1 h with Forskolin (30 μM) and IBMX (0.5 mM) (lanes 2 and 3), or treated for 16 h with paclitaxel (500 nM) (lanes 5 and 6), using Phospho-HP1γ (Ser83) Antibody (upper panel) or HP1γ Antibody #2619 (lower panel).



Confocal immunofluorescent analysis of HeLa cells, untreated (left), forskolin- and IBMX-treated (center) or λ phosphatase-treated (right), using Phospho-HP1γ (Ser83) Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).


Heterochromatin protein 1 (HP1) is a family of heterochromatic adaptor molecules involved in both gene silencing and higher order chromatin structure (1). All three HP1 family members (α, β, and γ) are primarily associated with centromeric heterochromatin; however, HP1β and γ also localize to euchromatic sites in the genome (2,3). HP1 proteins are approximately 25 kDa in size and contain a conserved amino-terminal chromodomain, followed by a variable hinge region and a conserved carboxy-terminal chromoshadow domain. The chromodomain facilitates binding to histone H3 tri-methylated at Lys9, a histone "mark" closely associated with centromeric heterochromatin (4,5). The variable hinge region binds both RNA and DNA in a sequence-independent manner (6). The chromoshadow domain mediates the dimerization of HP1 proteins, in addition to binding multiple proteins implicated in gene silencing and heterochromatin formation, including the SUV39H histone methyltransferase, the DNMT1 and DNMT3a DNA methyltransferases, and the p150 subunit of chromatin-assembly factor-1 (CAF1) (7-9). In addition to contributing to heterochromatin formation and propagation, HP1 and SUV39H are also found complexed with retinoblastoma (Rb) and E2F6 proteins, both of which function to repress euchromatic gene transcription in quiescent cells (10,11). HP1 proteins are subject to multiple types of post-translational modifications, including phosphorylation, acetylation, methylation, ubiquitination, and sumoylation, suggesting multiple means of regulation (12-14).

HP1γ is phosphorylated on Ser83 by protein kinase A (PKA) in vitro, and activation of PKA by forskolin and IBMX treatment leads to increased phosphorylation in vivo (14). Phosphorylation of HP1γ on Ser83 also increases during mitosis as demonstrated by the Phospho-HP1γ (Ser83) Antibody, which shows increased immunofluorescent staining in untreated mitotic cells and increased Western blot signal in lysates from cells arrested in mitosis by treatment with paclitaxel. Phosphorylation of Ser83 only occurs on a subpopulation of HP1γ found associated with euchromatin, specifically HP1γ bound to coding regions of active genes (14). 
This phosphorylation impairs the ability of HP1γ to silence transcription and may be a marker for transcription elongation (14).

12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
2062   Histone Deacetylase 1 (HDAC1) Antibody
2613   HP1β Antibody
2619   HP1γ Antibody
2623   HP1α/β (C7F11) Rabbit mAb
6883   SignalFire™ ECL Reagent
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
9309   Rb (4H1) Mouse mAb
9701   Phospho-Histone H3 (Ser10) Antibody
9711   Acetyl- and Phospho-Histone H3 (Lys9/Ser10) Antibody
9715   Histone H3 Antibody

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Phospho-HP1γ (Ser83) Antibody

Metabolic Reprogramming in Disease