#2532 AMPKα Antibody
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CSTコード | 包装 | 希望納入価格 (円) |
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#2532S | 100 μL | 56,000 | ログインすると国内在庫状況がご確認いただけます。
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#2532L | 300 μL | 128,000 |
感度 | 分子量 (kDa) | 抗体の由来 | 貯法 | |
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内在性 | 62 | Rabbit | -20℃ |
種交差性 (社内試験済) | |
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ヒト、マウス、ラット、ハムスター、サル | - |
ウェスタンブロッティング (1:1000)、免疫沈降 (1:100)
特異性・感度 | |
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内在性レベルのAMPKα タンパク質を検出します。触媒作用をもつサブユニットであるα-1、α-2 アイソフォーム両方を検出しますが、βあるいはγサブユニットは検出しません。 |
使用抗原 | |
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ヒトのAMPKα タンパク質のN末端領域 (合成ペプチド) |
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社内データ |
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※下記の社内データは、すべて2532 の推奨プロトコールで実験した結果です。
Western Blotting

Western blot analysis of extracts from HEK293 cells, untreated or oligomycin-treated (0.5μM) for the indicated times, using AMPKα Antibody.
Western Blotting

Western blot analysis of extracts from various cell lines using AMPK alpha Antibody
IP

Immunprecipitation of AMPK from untreated 3T3 cell extracts using AMPK antibody (Lane 1). Lane 2: No antibody control. Lane 3: Input control.
バックグラウンド |
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AMP-activated protein kinase (AMPK) is highly conserved from yeast to plants and animals and plays a key role in the regulation of energy homeostasis (1). AMPK is a heterotrimeric complex composed of a catalytic α subunit and regulatory β and γ subunits, each of which is encoded by two or three distinct genes (α1, 2; β1, 2; γ1, 2, 3) (2). The kinase is activated by an elevated AMP/ATP ratio due to cellular and environmental stress, such as heat shock, hypoxia, and ischemia (1). The tumor suppressor LKB1, in association with accessory proteins STRAD and MO25, phosphorylates AMPKα at Thr172 in the activation loop, and this phosphorylation is required for AMPK activation (3-5). AMPKα is also phosphorylated at Thr258 and Ser485 (for α1; Ser491 for α2). The upstream kinase and the biological significance of these phosphorylation events have yet to be elucidated (6). The β1 subunit is post-translationally modified by myristoylation and multi-site phosphorylation including Ser24/25, Ser96, Ser101, Ser108, and Ser182 (6,7). Phosphorylation at Ser108 of the β1 subunit seems to be required for the activation of AMPK enzyme, while phosphorylation at Ser24/25 and Ser182 affects AMPK localization (7). Several mutations in AMPKγ subunits have been identified, most of which are located in the putative AMP/ATP binding sites (CBS or Bateman domains). Mutations at these sites lead to reduction of AMPK activity and cause glycogen accumulation in heart or skeletal muscle (1,2). Accumulating evidence indicates that AMPK not only regulates the metabolism of fatty acids and glycogen, but also modulates protein synthesis and cell growth through EF2 and TSC2/mTOR pathways, as well as blood flow via eNOS/nNOS (1).
- Hardie, D.G. (2004) J Cell Sci 117, 5479-87.
- Carling, D. (2004) Trends Biochem Sci 29, 18-24.
- Hawley, S.A. et al. (1996) J Biol Chem 271, 27879-87.
- Lizcano, J.M. et al. (2004) EMBO J 23, 833-43.
- Shaw, R.J. et al. (2004) Proc Natl Acad Sci USA 101, 3329-35.
- Woods, A. et al. (2003) J Biol Chem 278, 28434-42.
- Warden, S.M. et al. (2001) Biochem J 354, 275-83.
使用例 |
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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
本製品は試験研究用です。