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#2239 EGF Receptor (1F4) Mouse mAb

CSTコード 包装
希望納入価格 (円)
国内在庫 i
2019年12月13日15時35分 現在
ご登録代理店情報 i
#2239S100 μL56,000

EGFR XP®モノクローナル抗体 | EGFR 製品一覧 | EGFR 抗体比較

感度分子量 (kDa)抗体の由来貯法
内在性175Mouse IgG1-20℃
種交差性 (社内試験済)
交差する可能性がある種 i


ヒト -
2239 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。



ウェスタンブロッティング (1:1000)


内在性レベルのEGF Receptor を検出します。他のErb ファミリータンパク質とは交差しません。
ヒトのEGF Receptor のC末端配列 (合成ペプチド)

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※下記の社内データは、すべて2239 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from the indicated cell lines. EGF Receptor(1F4) Mouse mAb specifically detects EGF receptors expressed in A431 and MDA-MD-468 cells, but does not cross-react with ErbB2, 3, and 4 proteins expressed in MDA-MD-453 and MCF-7 cells.


The epidermal growth factor (EGF) receptor is a transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Ligand binding results in receptor dimerization, autophosphorylation, activation of downstream signaling, internalization, and lysosomal degradation (1,2). Phosphorylation of EGF receptor (EGFR) at Tyr845 in the kinase domain is implicated in stabilizing the activation loop, maintaining the active state enzyme, and providing a binding surface for substrate proteins (3,4). c-Src is involved in phosphorylation of EGFR at Tyr845 (5). The SH2 domain of PLCγ binds at phospho-Tyr992, resulting in activation of PLCγ-mediated downstream signaling (6). Phosphorylation of EGFR at Tyr1045 creates a major docking site for the adaptor protein c-Cbl, leading to receptor ubiquitination and degradation following EGFR activation (7,8). The GRB2 adaptor protein binds activated EGFR at phospho-Tyr1068 (9). A pair of phosphorylated EGFR residues (Tyr1148 and Tyr1173) provide a docking site for the Shc scaffold protein, with both sites involved in MAP kinase signaling activation (2). Phosphorylation of EGFR at specific serine and threonine residues attenuates EGFR kinase activity. EGFR carboxy-terminal residues Ser1046 and Ser1047 are phosphorylated by CaM kinase II; mutation of either of these serines results in upregulated EGFR tyrosine autophosphorylation (10).

12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
2220   Phospho-EGF Receptor (Tyr1086) Antibody
2231   Phospho-EGF Receptor (Tyr845) Antibody
2232   EGF Receptor Antibody
2234   Phospho-EGF Receptor (Tyr1068) Antibody
2235   Phospho-EGF Receptor (Tyr992) Antibody
2236   Phospho-EGF Receptor (Tyr1068) (1H12) Mouse mAb
2237   Phospho-EGF Receptor (Tyr1045) Antibody
2238   Phospho-EGF Receptor (Ser1046/1047) Antibody
2256   EGF Receptor (EGFR1) Mouse mAb (IP Specific)
2641   Phospho-EGF Receptor (Tyr998) (C24A5) Rabbit mAb
2646   EGF Receptor (C74B9) Rabbit mAb
2963   EGF Receptor (T43) Antibody
3056   Phospho-EGF Receptor (Thr669) Antibody
3265   EGF Receptor (V279) Antibody
3777   Phospho-EGF Receptor (Tyr1068) (D7A5) XP® Rabbit mAb
4267   EGF Receptor (D38B1) XP® Rabbit mAb
4404   Phospho-EGF Receptor (Tyr1148) Antibody
4405   EGF Receptor (15F8) Rabbit mAb
4407   Phospho-EGF Receptor (Tyr1173) (53A5) Rabbit mAb
6883   SignalFire™ ECL Reagent
7076   Anti-mouse IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
8102   SignalSlide® Phospho-EGF Receptor IHC Controls
8112   SignalStain® Antibody Diluent
9922   Phospho-EGF Receptor Antibody Sampler Kit

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.


EGF Receptor (1F4) Mouse mAb

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