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#2083 Phospho-Raptor (Ser792) Antibody

CSTコード 包装
希望納入価格 (円)
国内在庫 i
2019年12月11日15時25分 現在
ご登録代理店情報 i
#2083S100 μL66,000
#2083T20 μL39,000
Custom Antibody Sampler Kitの構成品を選択できます。

RAPTOR 製品一覧 | #2083 が入っているAntibody Sampler キット一覧

感度分子量 (kDa)抗体の由来貯法
種交差性 (社内試験済)
交差する可能性がある種 i


ヒト、マウス、ラット -
2083 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。



ウェスタンブロッティング (1:1000)


内在性レベルのSer792 がリン酸化されたRaptor タンパク質を検出します。様々な分子量の非特異的なシグナルを検出する可能性があります。
ヒトのRaptor タンパク質由来の配列 (合成ペプチド)

ホモロジー (相同性) 検索をご希望の場合 >>>



※下記の社内データは、すべて2083 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of C2C12 or 293 cells, untreated or treated with AICAR (0.5 mM for 30 minutes) or oligomycin (0.5 μM for 30 minutes), using Phospho-Raptor (Ser792) Antibody (upper and lower left ) or Raptor Antibody #2280 (upper and lower right).

*Cross-reacting bands at 200 kDa.

Western Blotting

Western Blotting

Western blot analysis of wild-type (WT) and AMPKα1 and α2 knockout (KO) mouse embryonic fibroblasts (MEFs), untreated or treated with AICAR (2 mM for 1 hour), using Phospho-Raptor (Ser792) Antibody (upper) or Raptor Antibody #4978 (lower). (Image provided by Dr. Reuben Shaw, Salk Institute for Biological Studies).

*Cross-reacting bands at 60, 70 and 240 kDa


The regulatory associated protein of mTOR (Raptor) was identified as an mTOR binding partner that mediates mTOR signaling to downstream targets (1,2). Raptor binds to mTOR substrates, including 4E-BP1 and p70 S6 kinase, through their TOR signaling (TOS) motifs and is required for mTOR-mediated phosphorylation of these substrates (3,4). Binding of the FKBP12-rapamycin complex to mTOR inhibits the mTOR-raptor interaction, suggesting a mechanism for rapamycin's specific inhibition of mTOR signaling (5). This mTOR-raptor interaction and its regulation by nutrients and/or rapamycin is dependent on a protein called GβL (6). GβL is also part of the rapamycin-insensitive complex between mTOR and rictor (rapamycin-insensitive companion of mTOR), and may mediate rictor-mTOR signaling to downstream targets including PKCα (7). Furthermore, the rictor-mTOR complex has been identified as the previously elusive PDK2 responsible for the phosphorylation of Akt/PKB on Ser473, facilitating phosphorylation of Akt/PKB on Thr308 by PDK1 and required for the full activation of Akt/PKB (8).

Recently raptor has been identified as a direct substrate of the AMP-activated protein kinase (AMPK) (9). AMPK phosphorylates raptor on Ser722/Ser792 (9). This phosphorylation is essential for inhibition of the raptor-containing mTOR complex 1 (mTORC1) and induces cell cycle arrest when cells are stressed for energy (9). These findings suggest that raptor is a critical switch that correlates cell cycle progression with energy status.

1230   Phospho-mTOR (Ser2448) Blocking Peptide
12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
2114   Rictor (53A2) Rabbit mAb
2140   Rictor Antibody
2280   Raptor (24C12) Rabbit mAb
2610   PRAS40 Antibody
2640   Phospho-PRAS40 (Thr246) Antibody
2691   PRAS40 (D23C7) XP® Rabbit mAb
2971   Phospho-mTOR (Ser2448) Antibody
2972   mTOR Antibody
2974   Phospho-mTOR (Ser2481) Antibody
2976   Phospho-mTOR (Ser2448) (49F9) Rabbit mAb (IHC Specific)
2983   mTOR (7C10) Rabbit mAb
3227   GβL Antibody
3274   GβL (86B8) Rabbit mAb
5536   Phospho-mTOR (Ser2448) (D9C2) XP® Rabbit mAb
6883   SignalFire™ ECL Reagent
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
9864   mTOR Regulation Antibody Sampler Kit
9904   Rapamycin
9997   Tris Buffered Saline with Tween® 20 (TBST-10X)
9999   Nonfat Dry Milk

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Phospho-Raptor (Ser792) Antibody

Immune Cell Signaling Pathways