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#2056 PKCα Antibody

CSTコード 包装
希望納入価格 (円)
国内在庫 i
2020年2月17日11時40分 現在
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#2056S100 μL58,000
#2056T20 μL39,000
Custom Antibody Sampler Kitの構成品を選択できます。

PKCALPHA 製品一覧 | #2056 が入っているAntibody Sampler キット一覧

感度分子量 (kDa)抗体の由来貯法
種交差性 (社内試験済)
交差する可能性がある種 i


ヒト、マウス、ラット、サル イヌ
2056 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。



ウェスタンブロッティング (1:1000)免疫沈降 (1:50)免疫蛍光細胞染色 (IF-IC) (1:50)フローサイトメトリー (1:50)


内在性レベルのPKCαタンパク質を検出します。内在性レベルの他のPKC タンパク質のアイソフォームとは交差しません。
ヒトのPKCαタンパク質 (合成ペプチド)

ホモロジー (相同性) 検索をご希望の場合 >>>



※下記の社内データは、すべて2056 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts of HeLa, COS, C6 and NIH/3T3 cells, using PKCα Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts of Baculovirus expressed PKC isoforms demonstrating the isoform-specificity of PKCα Antibody.



Confocal immunofluorescent images of C6 cells serum-starved (left) or TPA #9905 treated (center), labeled with PKCα Antibody (green) compared to an isotype control (right). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells using PKCα Antibody (solid line) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed line). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.


Activation of protein kinase C (PKC) is one of the earliest events in a cascade that controls a variety of cellular responses, including secretion, gene expression, proliferation, and muscle contraction (1,2). PKC isoforms belong to three groups based on calcium dependency and activators. Classical PKCs are calcium-dependent via their C2 domains and are activated by phosphatidylserine (PS), diacylglycerol (DAG), and phorbol esters (TPA, PMA) through their cysteine-rich C1 domains. Both novel and atypical PKCs are calcium-independent, but only novel PKCs are activated by PS, DAG, and phorbol esters (3-5). Members of these three PKC groups contain a pseudo-substrate or autoinhibitory domain that binds to substrate-binding sites in the catalytic domain to prevent activation in the absence of cofactors or activators. Control of PKC activity is regulated through three distinct phosphorylation events. Phosphorylation occurs in vivo at Thr500 in the activation loop, at Thr641 through autophosphorylation, and at the carboxy-terminal hydrophobic site Ser660 (2). Atypical PKC isoforms lack hydrophobic region phosphorylation, which correlates with the presence of glutamic acid rather than the serine or threonine residues found in more typical PKC isoforms. The enzyme PDK1 or a close relative is responsible for PKC activation. A recent addition to the PKC superfamily is PKCμ (PKD), which is regulated by DAG and TPA through its C1 domain. PKD is distinguished by the presence of a PH domain and by its unique substrate recognition and Golgi localization (6). PKC-related kinases (PRK) lack the C1 domain and do not respond to DAG or phorbol esters. Phosphatidylinositol lipids activate PRKs, and small Rho-family GTPases bind to the homology region 1 (HR1) to regulate PRK kinase activity (7).

12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
2051   Phospho-PKD/PKCμ (Ser916) Antibody
2054   Phospho-PKD/PKCμ (Ser744/748) Antibody
2058   PKCδ Antibody
2261   Phospho-(Ser) PKC Substrate Antibody
6883   SignalFire™ ECL Reagent
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
9371   Phospho-PKC (pan) (βII Ser660) Antibody
9372   PKCζ Antibody
9374   Phospho-PKCδ (Thr505) Antibody
9375   Phospho-PKCα/β II (Thr638/641) Antibody
9376   Phospho-PKCδ/θ (Ser643/676) Antibody
9377   Phospho-PKCθ (Thr538) Antibody
9378   Phospho-PKCζ/λ (Thr410/403) Antibody
9379   Phospho-PKC (pan) (gamma Thr514) Antibody
9921   Phospho-PKC Antibody Sampler Kit

DRAQ5 is a registered trademark of Biostatus Limited.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.


PKCα Antibody

Immune Cell Signaling Pathways