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#14820 ATRX (D1N2E) Rabbit mAb

CSTコード 包装
希望納入価格 (円)
国内在庫 i
2019年11月15日15時30分 現在
ご登録代理店情報 i
#14820S100 μL56,000
感度分子量 (kDa)抗体の由来貯法
内在性280Rabbit IgG-20℃
種交差性 (社内試験済)
交差する可能性がある種 i


ヒト -
14820 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。



ウェスタンブロッティング (1:1000)免疫沈降 (1:50)クロマチン免疫沈降 (1:50)


内在性レベルのATRX タンパク質を検出します。
ヒトのATRX タンパク質のLeu1189 周辺領域 (合成ペプチド)

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※下記の社内データは、すべて14820 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using ATRX (D1N2E) Rabbit mAb (upper) and Nucleolin (D4C7O) Rabbit mAb #14574. As expected, the signal for ATRX is not present in the negative cell line U2OS.



Immunoprecipitation of ATRX from 293T cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or ATRX (D1N2E) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using ATRX (D1N2E) Rabbit mAb.

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells and either ATRX (D1N2E) Rabbit mAb or Normal Rabbit IgG #2729, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human 18s rDNA repeat primers, SimpleChIP® Human 28s rDNA Repeat Primers #14901, and SimpleChIP® Human GAPDH Exon 1 Primers #5516. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.


α-thalassemia/mental retardation X-linked (ATRX) is transcriptional regulator and helicase that belongs to the SNF2 family of chromatin remodeling proteins (1,2). Together with its binding partner death-associated protein 6 (DAXX), ATRX acts as histone chaperone to deposit histone variant H3.3 at repetitive DNA sequences such as telomeric, pericentric, and ribosomal gene repeats (3-6). ATRX is involved in many nuclear functions that ensure proper sister chromatid cohesion during mitosis and chromosome alignment during meiosis (7,8). The ATRX transcriptional regulator also plays a role in the maintenance of telomere integrity and the regulation of gene expression during mammalian development by influencing DNA methylation patterns at high DNA repeat sequences (9,10). Mutations in the corresponding ATRX gene results in ATR-X syndrome, an X-linked disorder characterized by intellectual disabilities, craniofacial abnormalities, and mild α-thalassemia (11,12). Research studies indicate that the loss of ATRX protein occurs in numerous cancers, including pancreatic neuroendocrine tumors (PanNETs) and pediatric glioblastoma, where telomere maintenance occurs independently of telomerase (13-16).

12757   SignalFire™ Elite ECL Reagent
14901   SimpleChIP® Human 28S rDNA Repeat Primers
4533   Daxx (25C12) Rabbit mAb
5516   SimpleChIP® Human GAPDH Exon 1 Primers
6883   SignalFire™ ECL Reagent
7017   6-Tube Magnetic Separation Rack
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
9002   SimpleChIP® Enzymatic Chromatin IP Kit (Agarose Beads)
9003   SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads)
9004   SimpleChIP® Plus Enzymatic Chromatin IP Kit (Agarose Beads)
9005   SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads)
9006   ChIP-Grade Protein G Magnetic Beads
9007   ChIP-Grade Protein G Agarose Beads

Tween is a registered trademark of ICI Americas, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
SimpleChIP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.


ATRX (D1N2E) Rabbit mAb

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