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#14111 Mono-Methyl-Histone H3 (Lys36) (D9J1D) Rabbit mAb

 
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希望納入価格 (円)
国内在庫 i
2019年11月15日15時30分 現在
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#14111S100 μL66,000
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#14111T20 μL39,000
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HISTONE H3 製品一覧 | #14111 が入っているAntibody Sampler キット一覧

感度分子量 (kDa)抗体の由来貯法
内在性17Rabbit IgG-20℃
種交差性 (社内試験済)
交差する可能性がある種 i

社内試験はしていませんが、配列が100%相同であるため反応すると推定される種

ヒト、マウス、ラット、サル ハムスター、ウシ
14111 の推奨プロトコール i

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注:各製品に最適化されたプロトコールをリンクしています。

 

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特異性・感度
内在性レベルのLys36 がモノメチル化されたHistone H3 タンパク質を検出します。Lys36 が非メチル化、ジメチル化、トリメチル化されたHistone H3 タンパク質とは交差しません。また、Lys4、Lys9、Lys27、Lys79 がモノメチル化されたHistone H3 タンパク質とも交差しません。
使用抗原
ヒトのHistone H3 タンパク質のモノメチル化Lys36 周辺領域 (合成ペプチド)

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社内データ

※下記の社内データは、すべて14111 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Mono-Methyl-Histone H3 (Lys36) (D9J1D) Rabbit mAb.

Western Blotting

Western Blotting

Antibody specificity was determined by western blotting. HeLa and NIH/3T3 cell extracts were probed with Mono-Methyl-Histone H3 (Lys36) (D9J1D) Rabbit mAb alone (A) or Mono-Methyl-Histone H3 (Lys36) (D9J1D) Rabbit mAb

pre-adsorbed with 1.5 μM of various competitor peptides (B-I). As shown, only the mono-methyl-histone H3 (Lys36)

peptide competed away binding of the antibody (C).

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using Mono-Methyl-Histone H3 (Lys36) (D9J1D) Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red).


Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells using Mono-Methyl-Histone H3 (Lys36) (D9J1D) Rabbit mAb (solid line) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed line). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody.

Chromatin IP-seq

Chromatin IP-seq

Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells and Mono-Methyl-Histone H3 (Lys36) (D9J1D) Rabbit mAb, using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA libraries were prepared using SimpleChIP® ChIP-seq DNA Library Prep Kit for Illumina® #56795. The figures show binding across WDR70 gene. For additional ChIP-seq tracks, please download the product data sheet.

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells and either Mono-Methyl-Histone H3 (Lys36) (D9J1D) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human AFM Intron 1 Primers #5098, SimpleChIP® Human GAPDH Exon 1 Primers #5516, SimpleChIP® Human MyoD Exon 1 Primers #4490, and SimpleChIP® Human MYT-1 Exon 1 Primers #4493. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.


バックグラウンド

The nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of chromatin. Originally thought to function as a static scaffold for DNA packaging, histones have now been shown to be dynamic proteins, undergoing multiple types of post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (1). Histone methylation is a major determinant for the formation of active and inactive regions of the genome and is crucial for the proper programming of the genome during development (2,3). Arginine methylation of histones H3 (Arg2, 17, 26) and H4 (Arg3) promotes transcriptional activation and is mediated by a family of protein arginine methyltransferases (PRMTs), including the co-activators PRMT1 and CARM1 (PRMT4) (4). In contrast, a more diverse set of histone lysine methyltransferases has been identified, all but one of which contain a conserved catalytic SET domain originally identified in the Drosophila Su(var)3-9, Enhancer of zeste, and Trithorax proteins. Lysine methylation occurs primarily on histones H3 (Lys4, 9, 27, 36, 79) and H4 (Lys20) and has been implicated in both transcriptional activation and silencing (4). Methylation of these lysine residues coordinates the recruitment of chromatin modifying enzymes containing methyl-lysine binding modules such as chromodomains (HP1, PRC1), PHD fingers (BPTF, ING2), tudor domains (53BP1), and WD-40 domains (WDR5) (5-8). The discovery of histone demethylases such as PADI4, LSD1, JMJD1, JMJD2, and JHDM1 has shown that methylation is a reversible epigenetic marker (9).

使用例
 
関連製品
2650   Histone H3 Antibody (ChIP Formulated)
2901   Di-Methyl-Histone H3 (Lys36) (C75H12) Rabbit mAb
4490   SimpleChIP® Human MyoD1 Exon 1 Primers
4493   SimpleChIP® Human MYT-1 Exon 1 Primers
4499   Histone H3 (D1H2) XP® Rabbit mAb
4620   Histone H3 (D2B12) XP® Rabbit mAb (ChIP Formulated)
5328   JMJD2A (C37E5) Rabbit mAb
5516   SimpleChIP® Human GAPDH Exon 1 Primers
7017   6-Tube Magnetic Separation Rack
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
8639   JMJD2B (D7E6) Rabbit mAb
9002   SimpleChIP® Enzymatic Chromatin IP Kit (Agarose Beads)
9003   SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads)
9004   SimpleChIP® Plus Enzymatic Chromatin IP Kit (Agarose Beads)
9005   SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads)
9006   ChIP-Grade Protein G Magnetic Beads
9007   ChIP-Grade Protein G Agarose Beads
9715   Histone H3 Antibody
9717   Histone H3 (3H1) Rabbit mAb
9763   Tri-Methyl-Histone H3 (Lys36) Antibody
9999   Nonfat Dry Milk

Illumina is a registered trademark of Illumina, Inc.
Tween is a registered trademark of ICI Americas, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
SimpleChIP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

Mono-Methyl-Histone H3 (Lys36) (D9J1D) Rabbit mAb

Immune Cell Signaling Pathways

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