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#13859 Cellular Glutathione Detection Assay Kit

 
  • Cellular Glutathione Detection Assay Kit Data Sheet PDF
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希望納入価格 (円)
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2019年8月20日15時55分 現在
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#13859S1 Kit66,000
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キット内容 容量
Reduced Glutathione Standard 1 x 1 ea
Glutathione-S-Transferase 1 x 1 ea
Tris Assay Buffer 1 x 25 ml
Digitonin Lysis Buffer 1 x 11 ml
Monochlorobimane 1 x 1 ea
Kit 情報
細胞透過性色素 (Monochlorobimane) を使用し、細胞内の還元型グルタチオンを検出します。
種交差性
すべての種
貯法
-20℃
※Tris Assay Buffer は保存温度が4℃となっておりますが、輸送時においては-20℃とさせて頂いています。本品受領後は速やかに冷蔵庫 (4℃) での保存をお願いいたします。
社内データ

Flow Cytometry

Flow Cytometry

Figure 5. Jurkat cells, untreated (green) or treated with terfenadine (200 μΜ, 4 hr; blue), were labeled with MCB (100 μΜ, 30 min) and analyzed using direct flow cytometry at an excitation wavelength of 380 nm and an emission wavelength of about 460 nm.

Fluorescent Detection

Fluorescent Detection

Figure 1. Reduced Glutathione Standard was diluted in Tris Assay Buffer and samples were assayed to create a standard curve. This standard curve is for demonstration purposes only; in order to accurately determine glutathione levels users should generate a standard curve for each sample set.

Fluorescent Detection

Fluorescent Detection

Figure 2. Raw 264.7, HeLa, and Jurkat cells were seeded in 96-well plates at various cell densities. Cellular glutathione levels were determined using the Cellular Glutathione Detection Assay Kit using both cell extracts (upper) and live cells (lower).


Fluorescent Detection

Fluorescent Detection

Figure 3. The relationship between the protein concentration of lysates from HeLa Cells, untreated and treated with Staurosporine #9953 (2.0 μΜ), and RFU as determined by the Cellular Glutathione Detection Assay Kit is shown.

Fluorescent Detection

Fluorescent Detection

Figure 4. HeLa cells (4x104 cells/well) were treated with terfenadine (indicated concentrations, 4 hr) and reduced glutathione levels were determined using the Cellular Glutathione Detection Assay Kit. Relative fluorescence units (RFU) were measured using a fluorescent plate reader with an excitation wavelength of 380 nm and an emission wavelength of about 460 nm.

Fluorescent Detection

Fluorescent Detection

Figure 6. Jurkat cells, untreated or terfenadine-treated (200 μΜ, 4 hr), were labeled with MCB (100 μΜ, 30 min) and Propidium Iodide (PI)/RNase Staining Solution #4087 and analyzed under a fluorescent microscope. Live cells (untreated) show high reduced-glutathione level (blue) while dead cells (treated) stained with PI (red) due to their compromised plasma membrane.


バックグラウンド

The antioxidant glutathione is found in both reduced and oxidized states in cells. Reduced glutathione can play an important role in preventing cellular damage caused by reactive oxygen species, including free radicals and peroxides. Reduced glutathione (GSH) acts as an electron donor in the presence of free radicals and peroxides to become oxidized (GSSG). GSH also participates in redox signaling through the removal of the cellular second messenger H2O2 (1,2). Diminished glutathione levels are observed during the aging process and in oxidative stress-related diseases. The depletion of GSH is necessary for the progression of apoptosis that is mediated by various signaling pathways (3,4). Intracellular GSH levels can be a very useful indicator for overall cell health, proliferation, and death (2).

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

Cellular Glutathione Detection Assay Kit

Immune Cell Signaling Pathways

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