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#13826 ERRα (E1G1J) Rabbit mAb

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希望納入価格 (円)
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2019年12月13日15時35分 現在
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#13826S100 μL56,000
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感度分子量 (kDa)抗体の由来貯法
内在性50Rabbit IgG-20℃
種交差性 (社内試験済)
交差する可能性がある種 i

社内試験はしていませんが、配列が100%相同であるため反応すると推定される種

ヒト、マウス、ラット、サル ウシ、イヌ、ブタ、ウマ
13826 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。

注:各製品に最適化されたプロトコールをリンクしています。

 

ウェスタンブロッティング (1:1000)クロマチン免疫沈降 (1:50)クロマチン免疫沈降シークエンス (1:50)

CST推奨プロトコールに欠かせない関連製品

特異性・感度
内在性レベルのERRαタンパク質を検出します。ERR ファミリータンパク質であるERRβとERRγとは交差しません。また、ERαタンパク質とERβタンパク質のどちらとも交差しません。
使用抗原
ヒトのERRαタンパク質のC末端近傍領域 (合成ペプチド)

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社内データ

※下記の社内データは、すべて13826 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using ERRα (E1G1J) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from BT-474 cells, untreated (-) or XCT790-treated (5 μM, 24 hr; +), using ERRα (E1G1J) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). XCT790 is a selective inverse agonist of ERRα that induces its proteasomal degradation (10,11).

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing Myc/DDK-tagged full-length human ERRα protein (hERRα-Myc/DDK; +), full-length human ERRβ protein (hERRβ-Myc/DDK; +); full-length human ERRγ protein (hERRγ-Myc/DDK; +), full-length human ERα protein (ERα-Myc/DDK; +), or full-length human ERβ protein (hERβ-Myc/DDK; +), using ERRα (E1G1J) Rabbit mAb (upper) and DYKDDDDK Tag Antibody #2368 (lower).


Western Blotting

Western Blotting

Western blot analysis of heart tissue extracts from ERRα wildtype (+/+) and ERRα knockout (-/-) mice using ERRα (E1G1J) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Heart tissue was kindly provided by Dr. Vincent Giguere of McGill University.

Chromatin IP-seq

Chromatin IP-seq

Chromatin immunoprecipitations were performed with cross-linked chromatin from Hepa 1-6 cells and ERRα (E1G1J) Rabbit mAb, using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Libraries were prepared using SimpleChIP® ChIP-seq DNA Library Prep Kit for Illumina® #56795. The figure shows binding across BAD gene. For additional ChIP-seq tracks, please download the product data sheet.

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from Hepa 1-6 cells and either ERRα (E1G1J) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using mouse ESRRA promoter primers, mouse IDH3A intron 2 primers, SimpleChIP® Mouse OGDH Intron 1 Primers #13828, and SimpleChIP® Mouse MYT-1 Promoter Primers #8985. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.


バックグラウンド

The estrogen-related receptor (ERR) subfamily of orphan nuclear receptors include three protein receptors, ERRα/NR3B1, ERRβ/NR3B2, and ERRγ/NR3B3, that have yet to be associated with natural ligands. PGC-1 coactivators regulate ERR transcription activation ability and receptor-induced transcription of genes involved in lipid metabolism, glucose metabolism, and mitochondrial biogenesis (1).

Estrogen-related receptor α (ERRα/NR3B1) is an orphan nuclear receptor that controls transcription of genes involved in fatty acid oxidation, glucose metabolism, and mitochondrial biogenesis (1,2). The receptor protein contains a non-conserved amino terminal domain (NTD), a central zinc finger DNA binding domain, and a ligand-binding domain. The carboxy-terminal AF2 helix motif of ERRα contains binding sites for nuclear receptor coactivators PGC-1α and PGC-1β (3-5). Research studies demonstrate that ERRα transcriptional activity is regulated through phosphorylation and sumoylation within the NTD (6). ERRα is ubiquitously expressed, with strong expression observed in heart, kidneys, skeletal muscle, and other high metabolic demand tissues (2). Additional studies indicate that ERRα is coexpressed in breast tumors with unfavorable biomarkers (7). The pharmacologic inhibition of ERRα activity in breast cancer might serve as a valuable therapeutic approach (8,9).

使用例
 
関連製品
12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
13258   Estrogen Receptor α (D6R2W) Rabbit mAb
13828   SimpleChIP® Mouse OGDH Intron 1 Primers
2729   Normal Rabbit IgG
6883   SignalFire™ ECL Reagent
7017   6-Tube Magnetic Separation Rack
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
8644   Estrogen Receptor α (D8H8) Rabbit mAb
8985   SimpleChIP® Mouse MYT-1 Promoter Primers
9002   SimpleChIP® Enzymatic Chromatin IP Kit (Agarose Beads)
9003   SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads)
9004   SimpleChIP® Plus Enzymatic Chromatin IP Kit (Agarose Beads)
9005   SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads)
9006   ChIP-Grade Protein G Magnetic Beads
9007   ChIP-Grade Protein G Agarose Beads
9997   Tris Buffered Saline with Tween® 20 (TBST-10X)
9999   Nonfat Dry Milk

Tween is a registered trademark of ICI Americas, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
SimpleChIP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

ERRα (E1G1J) Rabbit mAb

Metabolic Reprogramming in Disease

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