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#13421 SSRP1 (E1Y8D) Rabbit mAb

CSTコード 包装
希望納入価格 (円)
国内在庫 i
2019年11月21日15時20分 現在
ご登録代理店情報 i
#13421S100 μL56,000

SSRP1 製品一覧

感度分子量 (kDa)抗体の由来貯法
内在性81Rabbit IgG-20℃
種交差性 (社内試験済)
交差する可能性がある種 i


ヒト、マウス、ラット、サル ニワトリ
13421 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。



ウェスタンブロッティング (1:1000)クロマチン免疫沈降 (1:50)


内在性レベルのSSRP1 タンパク質を検出します。いくつかの細胞株で由来不明の140 kDa のタンパク質とも交差します。
ヒトのSSRP1 タンパク質のVal145 周辺領域 (合成ペプチド)

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※下記の社内データは、すべて13421 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using SSRP1 (E1Y8D) Rabbit mAb.

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from HCT 116 cells starved for 48 hr then serum stimulated with 20% FBS for 15 min and either SSRP1 (E1Y8D) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human c-Fos Promoter Primers #4663, SimpleChIP® Human c-Fos Exon 3 Primers #12010, SimpleChIP® Human EGR1 Promoter Primers #5549, and SimpleChIP® Human EGR1 Intron 3 Primers #11953. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.


Suppressor of Ty-16 (SPT16) and structure-specific recognition protein-1 (SSRP1) are subunits of the facilitates chromatin transcription (FACT) complex that is essential for transcription elongation (1,2). FACT facilitates RNA polymerase-dependent transcription of chromatin templates by destabilizing the nucleosomes within the open reading frames of active genes (3-5). FACT destabilizes the nucleosomes, which would otherwise act as barriers to RNA polymerase transcription activity, by disrupting histone-histone and histone-DNA contacts that lead to the eviction of the histone H2A-H2B dimer (2,3,6). FACT may also function as a histone chaperone to reassemble nucleosomes after RNA polymerase passage (7). In addition to transcription, FACT activity has been shown to have a role in DNA replication in yeast and in DNA repair by contributing to the activation of p53 by CK2 and by facilitating histone H2AX-H2B exchange upon DNA damage (8-10).

11953   SimpleChIP® Human EGR1 Intron 3 Primers
12010   SimpleChIP® Human c-Fos Exon 3 Primers
12191   SPT16 (D7I2K) Rabbit mAb
12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
4663   SimpleChIP® Human c-Fos Promoter Primers
5549   SimpleChIP® Human EGR1 Promoter Primers
6883   SignalFire™ ECL Reagent
7017   6-Tube Magnetic Separation Rack
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
9002   SimpleChIP® Enzymatic Chromatin IP Kit (Agarose Beads)
9003   SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads)
9004   SimpleChIP® Plus Enzymatic Chromatin IP Kit (Agarose Beads)
9005   SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads)
9006   ChIP-Grade Protein G Magnetic Beads
9007   ChIP-Grade Protein G Agarose Beads

Tween is a registered trademark of ICI Americas, Inc.
SimpleChIP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.


SSRP1 (E1Y8D) Rabbit mAb

Immune Cell Signaling Pathways