#13054 DyLight™ 554 Phalloidin
|Phalloidin とF-Actin との結合を通して固定細胞の細胞骨格を蛍光標識します。Phalloidin は毒性があるため、生細胞では使用しません。|
|免疫蛍光染色 (IF-IC、IF-F) (1:200)|
Confocal immunofluorescent analysis of SK-N-AS cells using Cox IV (4D11-B3-E8) Mouse mAb #11967 (green). Actin filaments were labeled with DyLight™ 554 Phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Actin is a ubiquitous eukaryotic protein and a major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells to control cell structure and motility (1). Actin exists mainly as a fibrous polymer called F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin resulting in an increase in the monomeric globular G-actin form (2). Phalloidin is a naturally occurring toxic bicyclic peptide found in the deathcap toadstool, Amanita phalloides, which rapidly binds to F-actin with strong affinity (3).
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