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#12146 Phospho-NEDD4L (Ser342) (D16D6) Rabbit mAb

CSTコード 包装
希望納入価格 (円)
国内在庫 i
2020年1月24日15時25分 現在
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#12146S100 μL67,000

NEDD4L 製品一覧

感度分子量 (kDa)抗体の由来貯法
内在性110,135Rabbit IgG-20℃
種交差性 (社内試験済)
交差する可能性がある種 i


ヒト、マウス、ラット -
12146 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。



ウェスタンブロッティング (1:1000)免疫沈降 (1:100)


内在性レベルのSer342 がリン酸化されたNEDD4L タンパク質を検出します。
ヒトのNEDD4L タンパク質のSer342 周辺領域 (合成リン酸化ペプチド)

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※下記の社内データは、すべて12146 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from SK-MEL-28 cells, untreated (-) or CIP-treated (+), using Phospho-NEDD4L (Ser342) (D16D6) Rabbit mAb (upper) or NEDD4L Antibody #4013 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from of HeLa, SK-MEL-28, and NIH/3T3 cells using Phospho-NEDD4L (Ser342) (D16D6) Rabbit mAb.


Neural precursor expressed, developmentally down-regulated protein 4 (NEDD4) was originally identified as a gene that is highly expressed in the early mouse embryonic central nervous system (1). Subsequently, a family of NEDD4-like proteins have been defined that includes seven members in humans (2). NEDD4 and NEDD4-like (NEDD4L) proteins contain multiple functional domains including a calcium-dependent phospholipid and membrane binding domain (C2 domain), two to four protein binding domains (WW domains), and an E3 ubiquitin-protein ligase domain (HECT domain). NEDD4 and NEDD4L have been shown to downregulate both neuronal voltage-gated Na+ channels (NaVs) and epithelial Na+ channels (ENaCs) in response to increased intracellular Na+ concentrations (3,4). The WW domains of NEDD4 bind to PY motifs (amino acid sequence PPXY) found in multiple NaV and ENaC proteins; ubiquitination of these proteins is mediated by the HECT domain of NEDD4 and results in their internalization and removal from the plasma membrane. Research studies have shown that mutation of the PY motifs in ENaC proteins is associated with Liddle's syndrome, an autosomal dominant form of hypertension (5). In addition to targeting sodium channels, NEDD4L has also been shown to negatively regulate TGF-β signaling by targeting Smad2 for degradation (6). Mouse and human NEDD4 are rapidly cleaved by caspase proteins during apoptosis, although the significance of this cleavage is not clear (7).

NEDD4L can be phosphorylated at Ser342 and Ser448 by several AGC kinase family members including SGK1, Akt, and PKA (8-11). Phosphorylation at these sites inhibits NEDD4L function as a suppressor of ENaCs and as a regulator of TGF-β signaling through its effects on Smad2/3 binding (8-11).

12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
4013   NEDD4L Antibody
6883   SignalFire™ ECL Reagent
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
8063   Phospho-NEDD4L (Ser448) Antibody

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Phospho-NEDD4L (Ser342) (D16D6) Rabbit mAb

Metabolic Reprogramming in Disease